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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGLL Recombinant antibody (86087-2-RR)

    WB analysis using 86087-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86087-2-RR (MGLL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using MGLL Recombinant antibody (86087-2-RR)

    WB analysis of human peripheral blood platelets using 86087-2-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 86087-2-RR (MGLL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86087-2-RR

    Affinity and Kinetic Characterization of 86087-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86087-2-RR against Human MGLL were performed. The affinity constant is 1.24 nM.

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MGLL Recombinant antibody

MGLL Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86087-2-RR
Clone No.250509G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:3.1.1.23, HU K5, HUK5, HU-K5, Monoacylglycerol lipase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, human peripheral blood platelets, U-87 MG cells, SMMC-7721 cells, PC-3 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86087-2-RR targets MGLL in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MGLL fusion protein Ag6970 相同性解析による交差性が予測される生物種
Full Name monoglyceride lipase
Calculated molecular weight 33 kDa
Observed molecular weight33-35 kDa
GenBank accession numberBC000551
Gene Symbol MGLL
Gene ID (NCBI) 11343
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain

Protocols

Product Specific Protocols
WB protocol for MGLL antibody 86087-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols