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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGLL Recombinant antibody (86087-2-RR)

    WB analysis using 86087-2-RR (same clone as 86087-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86087-2-RR (MGLL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86087-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using MGLL Recombinant antibody (86087-2-RR)

    WB analysis of human peripheral blood platelets using 86087-2-RR (same clone as 86087-2-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 86087-2-RR (MGLL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86087-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86087-2-RR

    Affinity and Kinetic Characterization of 86087-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86087-2-RR against Human MGLL were performed. The affinity constant is 1.24 nM.

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MGLL Recombinant antibody, PBS Only

MGLL Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 86087-2-PBS
Clone No.250509G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:3.1.1.23, HU K5, HUK5, HU-K5, Monoacylglycerol lipase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86087-2-PBS targets MGLL in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MGLL fusion protein Ag6970 相同性解析による交差性が予測される生物種
Full Name monoglyceride lipase
Calculated molecular weight 33 kDa
Observed molecular weight33-35 kDa
GenBank accession numberBC000551
Gene Symbol MGLL
Gene ID (NCBI) 11343
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99685
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain