Validation Data Gallery

Filter: AllWBIHC

WB analysis of human kidney using 17661-1-AP (same clone as 17661-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
WB analysis of rat lung using 17661-1-AP (same clone as 17661-1-PBS)
rat lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 17661-1-AP (same clone as 17661-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 17661-1-AP (same clone as 17661-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17661-1-AP (MFAP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

MFAP4 Polyclonal antibody, PBS Only

MFAP4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17661-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Microfibril-associated glycoprotein 4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17661-1-PBS targets MFAP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11920 Product name: Recombinant human MFAP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC062415 Sequence: MKALLALPLLLLLSTPPCAPQVSGIRGDALERFCLQQPLDCDDIYAQGYQSDGVYLIYPSGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWNDYKLGFGRADGEYWLGLQNMHLLTLKQKYELRVDLEDFENNTAYAKYADFSISPNAVSAEEDGYTLFVAGFEDGGVGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA 相同性解析による交差性が予測される生物種
Full Name	microfibrillar-associated protein 4
Calculated molecular weight	255 aa, 29 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC062415
Gene Symbol	MFAP4
Gene ID (NCBI)	4239
RRID	AB_2142355
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55083
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human kidney using 17661-1-AP (same clone as 17661-1-PBS)

WB analysis of rat lung using 17661-1-AP (same clone as 17661-1-PBS)

WB analysis of mouse heart using 17661-1-AP (same clone as 17661-1-PBS)

WB analysis of mouse lung using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human placenta using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human placenta using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human testis using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human testis using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human skin using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human skin using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human spleen using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human spleen using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)

IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)

MFAP4 Polyclonal antibody, PBS Only

MFAP4 Polyclonal Antibody for WB, IHC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information