Validation Data Gallery
Tested Applications
| Positive WB detected in | human kidney tissue, mouse heart tissue, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human lung tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
17661-1-AP targets MFAP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11920 Product name: Recombinant human MFAP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC062415 Sequence: MKALLALPLLLLLSTPPCAPQVSGIRGDALERFCLQQPLDCDDIYAQGYQSDGVYLIYPSGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWNDYKLGFGRADGEYWLGLQNMHLLTLKQKYELRVDLEDFENNTAYAKYADFSISPNAVSAEEDGYTLFVAGFEDGGVGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA 相同性解析による交差性が予測される生物種 |
| Full Name | microfibrillar-associated protein 4 |
| Calculated molecular weight | 255 aa, 29 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC062415 |
| Gene Symbol | MFAP4 |
| Gene ID (NCBI) | 4239 |
| RRID | AB_2142355 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55083 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MFAP4 antibody 17661-1-AP | Download protocol |
| WB protocol for MFAP4 antibody 17661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Thorax A large lung gene expression study identifying fibulin-5 as a novel player in tissue repair in COPD. | ||
Front Pharmacol MFAP4 is a novel prognostic biomarker in glioma correlating with immunotherapy resistance and ferroptosis | ||
J Proteome Res Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples. | ||
J Proteomics FOXL1 + Telocytes in mouse colon orchestrate extracellular matrix biodynamics and wound repair resolution | ||
BMC Biol The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis |
