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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated MEK6 Polyclonal antibody

MEK6 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-12745

Synonyms

MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, PRKMK6, SAPKK3

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL647-12745

    FC experiment of HeLa using CL647-12745

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MEK6 (CL647-12745) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12745-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-12745 targets MEK6 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK6 fusion protein Ag3706 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 6
Calculated molecular weight 334 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC012009
Gene symbol MAP2K6
Gene ID (NCBI) 5608
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 MEK6 antibody CL647-12745Download protocol
Standard Protocols
Click here to view our Standard Protocols