CoraLite® Plus 488-conjugated MED4 Monoclonal antibody
MED4 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2E6F4
Cat no : CL488-67839
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67839-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67839 targets MED4 in FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.
| Tested Reactivity | Human, Mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MED4 fusion protein Ag8562 相同性解析による交差性が予測される生物種 |
| Full Name | mediator complex subunit 4 |
| Calculated molecular weight | 270 aa, 30 kDa |
| Observed molecular weight | ~36 kDa |
| GenBank accession number | BC005189 |
| Gene symbol | MED4 |
| Gene ID (NCBI) | 29079 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 MED4 antibody CL488-67839 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
