Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-FcA98044 targets MCP-1/CCL2 in FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg31375 Product name: Recombinant Human MCP-1/CCL2 protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 24-99 aa of BC009716 Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-C motif) ligand 2 |
| Calculated molecular weight | 99 aa, 11 kDa |
| GenBank accession number | BC009716 |
| Gene Symbol | MCP-1/CCL2 |
| Gene ID (NCBI) | 6347 |
| RRID | AB_3746884 |
| Conjugate | Biotin |
| Excitation/Emission maxima wavelengths | - |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13500 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Monocyte chemoattractant protein-1 (MCP-1), also known as CCL2, is a chemokine that regulates monocyte and macrophage migration and infiltration. In addition to monocytes and macrophages, MCP-1 can also serve as a chemoattractant for T cells, B cells, NK cells, dendritic cells, and neutrophils. MCP-1 expression is typically induced by the release of inflammatory cytokines and other stimuli from sites of tissue injury and infection. It in turn promotes the extravasation of immune cells across blood vessel walls and their subsequent infiltration of the infection site. It may also play a role in macrophage polarization as well as the differentiation of monocytes into dendritic cells, MSDCs, and osteoclasts. Increased MCP-1 expression has been linked to inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and multiple sclerosis. (PMID: 19441883, 31921102)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin MCP-1/CCL2 antibody Biotin-FcA98044 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |