Validation Data Gallery
Product Information
The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.
| Description | The MBP-Trap Agarose Kit contains MBP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of MBP-fusion proteins and their interacting factors.
• Immunoprecipitation of low abundant MBP-fusion proteins • Significantly higher sensitivity than amylose resin and other affinity resins • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | Maltose-binding protein (MBP) of Escherichia coli |
| Binding capacity | 45 μg (0.625 nmol) of recombinant MBP-tagged protein (~74.2 kDa) per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 4 nM |
| Compatibility with mass spectrometry | The MBP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631382 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| MBP-Trap Agarose beads | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| mbtak_SDS_MBP-Trap Agarose Kit (EN) |
| Datasheet |
|---|
| Protocol MBP-Trap Agarose Kit (PDF) |
| Whitepaper |
|---|
| Immunoprecipitation of GST- and MBP-fusion proteins (PDF) |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |