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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MBNL1 Monoclonal antibody

MBNL1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H2C12

Cat no : CL488-66837

Synonyms

DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL, MBNL1, muscleblind like (Drosophila), Muscleblind like protein 1

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL488-66837

    IF Staining of A431 using CL488-66837

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MBNL1 antibody (CL488-66837, Clone: 1H2C12 ) at dilution of 1:500.

Tested Applications

Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MBNL1 Ready-To-Use IHC Kit. MBNL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66837-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

CL488-66837 targets MBNL1 in IF applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MBNL1 fusion protein Ag7752 相同性解析による交差性が予測される生物種
Full Name muscleblind-like (Drosophila)
Calculated molecular weight 42 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC050535
Gene symbol MBNL1
Gene ID (NCBI) 4154
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MBNL1 (muscleblind-like 1) is one of the MBNL proteins containing 3 members (MBNL1-3) in vertebrates. And MBNL1 is widely expressed in adult tissues including brain, heart and skeletal muscle (PMID:25274774). As a highly conserved RNA-binding protein, MBNL1 regulates alternative splicing, alternative polyadenylation, mRNA stability and mRNA localization in the nucleus. The neurological diseases DM1 and DM2 (myotonic dystrophy type 1 and type2) are caused by expansion of repetitive sequences in non-coding regions in the DMPK gene because of the inappropriate patterns of alternative splicing owing to MBNL1(PMID:24354850). The antibody could recognize the common full-length protein in applications.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MBNL1 antibody CL488-66837Download protocol
Standard Protocols
Click here to view our Standard Protocols