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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MATR3 Monoclonal antibody (68280-1-Ig)

    WB analysis using 68280-1-Ig (same clone as 68280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68280-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MATR3 Monoclonal antibody (68280-1-Ig)

    WB analysis using 68280-1-Ig (same clone as 68280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68280-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MATR3 Monoclonal antibody (68280-1-Ig)

    IF Staining of HEK-293 using 68280-1-Ig (same clone as 68280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (68280-1-Ig, Clone: 3B12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68280-1-PBS in a different storage buffer formulation.

MATR3 Monoclonal antibody, PBS Only

MATR3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68280-1-PBS
Clone No.3B12C7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Matrin-3, matrin 3, KIAA0723

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68280-1-PBS targets MATR3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag31502

Product name: Recombinant human MATR3 protein

Source: e coli.-derived, pET28a-SUMO

Tag: his

Domain: 495-847 aa of BC015031

Sequence: GRVIHLSNLPHSGYSDSAVLKLAEPYGKIKNYILMRMKSQAFIEMETREDAMAMVDHCLKKALWFQGRCVKVDLSEKYKKLVLRIPNRGIDLLKKDKSRKRSYSPDGKESPSDKKSKTDGSQKTESSTEGKEQEEKSGEDGEKDTKDDQTEQEPNMLLESEDELLVDEEEAAALLESGSSVGDETDLANLGDVASDGKKEPSDKAVKKDGSASAAAKKKLKKVDKIEELDQENEAALENGIKNEENTEPGAESSENADDPNKDTSENADGQSDENKDDYTIPDEYRIGPYQPNVPVGIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKKET

相同性解析による交差性が予測される生物種
Full Name matrin 3
Calculated molecular weight 847 aa, 95 kDa
Observed molecular weight120 kDa
GenBank accession numberBC015031
Gene Symbol MATR3
Gene ID (NCBI) 9782
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP43243
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

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