Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, rat brain tissue, A431 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, mouse brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68280-1-Ig targets MATR3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31502 Product name: Recombinant human MATR3 protein Source: e coli.-derived, pET28a-SUMO Tag: his Domain: 495-847 aa of BC015031 Sequence: GRVIHLSNLPHSGYSDSAVLKLAEPYGKIKNYILMRMKSQAFIEMETREDAMAMVDHCLKKALWFQGRCVKVDLSEKYKKLVLRIPNRGIDLLKKDKSRKRSYSPDGKESPSDKKSKTDGSQKTESSTEGKEQEEKSGEDGEKDTKDDQTEQEPNMLLESEDELLVDEEEAAALLESGSSVGDETDLANLGDVASDGKKEPSDKAVKKDGSASAAAKKKLKKVDKIEELDQENEAALENGIKNEENTEPGAESSENADDPNKDTSENADGQSDENKDDYTIPDEYRIGPYQPNVPVGIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKKET 相同性解析による交差性が予測される生物種 |
| Full Name | matrin 3 |
| Calculated molecular weight | 847 aa, 95 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC015031 |
| Gene Symbol | MATR3 |
| Gene ID (NCBI) | 9782 |
| RRID | AB_2935361 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43243 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MATR3 antibody 68280-1-Ig | Download protocol |
| WB protocol for MATR3 antibody 68280-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
