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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAST2 Polyclonal antibody (33335-1-AP)

    WB analysis using 33335-1-AP (same clone as 33335-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33335-1-AP (MAST2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MAST2 Polyclonal antibody (33335-1-AP)

    IF Staining of A431 using 33335-1-AP (same clone as 33335-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAST2 antibody (33335-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAST2 Polyclonal antibody (33335-1-AP)

    IF Staining of U2OS using 33335-1-AP (same clone as 33335-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MAST2 antibody (33335-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33335-1-PBS in a different storage buffer formulation.

MAST2 Polyclonal antibody, PBS Only

MAST2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 33335-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.11.1, KIAA0807, MAST205, Microtubule-associated serine/threonine-protein kinase 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33335-1-PBS targets MAST2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35818

Product name: Recombinant human MAST2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1480-1798 aa of NM_015112.2

Sequence: TLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRDPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLENTTPAQPKNLSPREQGKTQPPSAPRLAHPSYEDPSQGWLWESECAQAVKEDPALSITQVPDASGDRRQDVPCRGCPLTQKSEPSLRRGQEPGGHQKHRDLALVPDELLKQT

相同性解析による交差性が予測される生物種
Full Name microtubule associated serine/threonine kinase 2
Calculated molecular weight196kDa，1798aa
Observed molecular weight196 kDa
GenBank accession numberNM_015112.2
Gene Symbol MAST2
Gene ID (NCBI) 23139
RRIDAB_3742944
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6P0Q8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAST2 is a microtubule-associated serine/threonine kinase, belonging to an evolutionarily conserved branch of AGC protein kinase superfamily. Since its discovery in 1993, it has been widely expressed in many tissues, including brain, heart, lung, liver, intestine and kidney. MAST2 is associated with many diseases, such as breast cancer, liver cancer, myeloma, inflammatory bowel disease, cystic fibrosis and various neurological diseases.

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