Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IF/ICC detected in | U2OS cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33335-1-AP targets MAST2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35818 Product name: Recombinant human MAST2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1480-1798 aa of NM_015112.2 Sequence: TLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRDPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLENTTPAQPKNLSPREQGKTQPPSAPRLAHPSYEDPSQGWLWESECAQAVKEDPALSITQVPDASGDRRQDVPCRGCPLTQKSEPSLRRGQEPGGHQKHRDLALVPDELLKQT 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule associated serine/threonine kinase 2 |
| Calculated molecular weight | 196kDa，1798aa |
| Observed molecular weight | 196 kDa |
| GenBank accession number | NM_015112.2 |
| Gene Symbol | MAST2 |
| Gene ID (NCBI) | 23139 |
| RRID | AB_3742944 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6P0Q8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAST2 is a microtubule-associated serine/threonine kinase, belonging to an evolutionarily conserved branch of AGC protein kinase superfamily. Since its discovery in 1993, it has been widely expressed in many tissues, including brain, heart, lung, liver, intestine and kidney. MAST2 is associated with many diseases, such as breast cancer, liver cancer, myeloma, inflammatory bowel disease, cystic fibrosis and various neurological diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAST2 antibody 33335-1-AP | Download protocol |
| WB protocol for MAST2 antibody 33335-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |