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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP6/STOP Polyclonal antibody (25717-1-AP)

    WB analysis using 25717-1-AP (same clone as 25717-1-PBS)

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAP6/STOP Polyclonal antibody (25717-1-AP)

    WB analysis using 25717-1-AP (same clone as 25717-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MAP6/STOP Polyclonal antibody (25717-1-AP)

    IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MAP6/STOP Polyclonal antibody (25717-1-AP)

    IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using MAP6/STOP Polyclonal antibody (25717-1-AP)

    IF Staining of PC-12 using 25717-1-AP (same clone as 25717-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using MAP6/STOP antibody (25717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

MAP6/STOP Polyclonal antibody, PBS Only

MAP6/STOP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25717-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25717-1-PBS targets MAP6/STOP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22471

Product name: Recombinant human MAP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-484 aa of BC139780

Sequence: GQGPMVQEPLKKQGSVVPGPPKDLGPMIPLPVKDQDHTVPEPLKNESPVISAPVKDQGPSVPVPPKNQSPMVPAKVKDQGSVVPESLKDQGPRIPEPVKNQAPMVPAPVKDEGPMVSASVKDQGPMVSAPVKDQGPIVPAPVKGEGPIVPAPVKDEGPMVSAPIKDQDPMVPEHPKDESAMATAPIKNQGSMVSEPVKNQGLVVSGPVKDQDVVVPEHAKVHDSAVVAPVKNQGPVVPESVKNQDPILPVLVKDQGPTVLQPPKNQGRIVPEPLKNQVPIVPVPLKDQDPLVPVPAKDQGPAVPEPLKTQGPRDPQLPTVSPLPRVMIPTAPHTEYIESSP

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 6
Calculated molecular weight 813 aa, 87 kDa
Observed molecular weight120 kDa
GenBank accession numberBC139780
Gene Symbol MAP6
Gene ID (NCBI) 4135
RRIDAB_3085819
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JE9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAP6, also named STOP protein, has been isolated from brain preparations but is also present in other tissues such as the skeletal muscles and heart. MAP6 is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization. Different isoforms of STOP protein arise from alternative mRNA splicing and transcription initiation sites using a single gene. The neuronal MAP6 isoforms N-STOP (120 kDa) and E-STOP (80 kDa), the astrocyte MAP6 isoform A-STOP (60 KDa), and a fibroblastic MAP6 isoform F-STOP (48 kDa) have been identified.

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