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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP6/STOP Polyclonal antibody (25717-1-AP)

    WB analysis using 25717-1-AP

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAP6/STOP Polyclonal antibody (25717-1-AP)

    WB analysis using 25717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MAP6/STOP Polyclonal antibody (25717-1-AP)

    IHC staining of mouse cerebellum using 25717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MAP6/STOP Polyclonal antibody (25717-1-AP)

    IHC staining of mouse cerebellum using 25717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using MAP6/STOP Polyclonal antibody (25717-1-AP)

    IF Staining of PC-12 using 25717-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using MAP6/STOP antibody (25717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MAP6/STOP Polyclonal antibody

MAP6/STOP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25717-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA1878, MAP 6, MAP6, MAP6/STOP, MTAP6, N STOP, Stable tubule only polypeptide, STOP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse retina tissue, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25717-1-AP targets MAP6/STOP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22471

Product name: Recombinant human MAP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-484 aa of BC139780

Sequence: GQGPMVQEPLKKQGSVVPGPPKDLGPMIPLPVKDQDHTVPEPLKNESPVISAPVKDQGPSVPVPPKNQSPMVPAKVKDQGSVVPESLKDQGPRIPEPVKNQAPMVPAPVKDEGPMVSASVKDQGPMVSAPVKDQGPIVPAPVKGEGPIVPAPVKDEGPMVSAPIKDQDPMVPEHPKDESAMATAPIKNQGSMVSEPVKNQGLVVSGPVKDQDVVVPEHAKVHDSAVVAPVKNQGPVVPESVKNQDPILPVLVKDQGPTVLQPPKNQGRIVPEPLKNQVPIVPVPLKDQDPLVPVPAKDQGPAVPEPLKTQGPRDPQLPTVSPLPRVMIPTAPHTEYIESSP

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 6
Calculated molecular weight 813 aa, 87 kDa
Observed molecular weight120 kDa
GenBank accession numberBC139780
Gene Symbol MAP6
Gene ID (NCBI) 4135
RRIDAB_3085819
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JE9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP6, also named STOP protein, has been isolated from brain preparations but is also present in other tissues such as the skeletal muscles and heart. MAP6 is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization. Different isoforms of STOP protein arise from alternative mRNA splicing and transcription initiation sites using a single gene. The neuronal MAP6 isoforms N-STOP (120 kDa) and E-STOP (80 kDa), the astrocyte MAP6 isoform A-STOP (60 KDa), and a fibroblastic MAP6 isoform F-STOP (48 kDa) have been identified.

Protocols

Product Specific Protocols
IF protocol for MAP6/STOP antibody 25717-1-APDownload protocol
IHC protocol for MAP6/STOP antibody 25717-1-APDownload protocol
WB protocol for MAP6/STOP antibody 25717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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