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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated MAP2K4 Monoclonal antibody

MAP2K4 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E4C1

Cat no : CL594-67333

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-67333

    IF Staining of HepG2 using CL594-67333

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 MAP2K4 antibody (CL594-67333, Clone: 2E4C1 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-67333

    FC experiment of HepG2 using CL594-67333

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2K4 (CL594-67333, Clone:2E4C1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67333-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67333 targets MAP2K4 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAP2K4 fusion protein Ag11348 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 4
Calculated molecular weight 399 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC036032
Gene symbol MAP2K4
Gene ID (NCBI) 6416
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 MAP2K4 antibody CL594-67333Download protocol
FC protocol for CL594 MAP2K4 antibody CL594-67333Download protocol
Standard Protocols
Click here to view our Standard Protocols