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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP2 Polyclonal antibody (17490-1-AP)

    WB analysis using 17490-1-AP (same clone as 17490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    WB analysis of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    WB analysis of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IP experiment of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    IP result of anti-MAP2 (IP:17490-1-AP, 3ug; Detection:17490-1-AP 1:1000) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IP experiment of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    IP result of anti-MAP2 (IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:8000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using MAP2 Polyclonal antibody (17490-1-AP)

    IP experiment of SH-SY5Y using 17490-1-AP (same clone as 17490-1-PBS)

    IP result of anti-MAP2 (IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:10000) with SH-SY5Y cells lysate 1240 ug. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IHC staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IHC staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IHC staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of iPS cells using MAP2 Polyclonal antibody (17490-1-AP)

    IF Staining of iPS cells using 17490-1-AP (same clone as 17490-1-PBS)

    Immunofluorescent staining of MAP2 (17490-1-AP, 1:250 dilution) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (RED MAP2; Blue: DAPI). Provided by BioTalentum Ltd., Hungary. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Neuro-2a cells using MAP2 Polyclonal antibody (17490-1-AP)

    FC experiment of Neuro-2a using 17490-1-AP (same clone as 17490-1-PBS)

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (17490-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

MAP2 Polyclonal antibody, PBS Only

MAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
Cat No. 17490-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Synonyms

MAP 2, MAP-2, MAP2A, MAP2B, MAP2C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17490-1-PBS targets MAP2 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11580

Product name: Recombinant human MAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 213-559 aa of BC038857

Sequence: TSAGSTDRLPYSKSGNKDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVRILNKKIDFSKVQSRCGSKDNIKHSAGGGNVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 2
Calculated molecular weight 200 kDa
Observed molecular weight 280 kDa, 70-85 kDa
GenBank accession numberBC038857
Gene Symbol MAP2
Gene ID (NCBI) 4133
RRIDAB_2137880
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11137
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.

What is the molecular weight of MAP2? Is MAP2 post-translationally modified?

MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms).

What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2?

MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells.

Can MAP2 be used as a neuronal marker?

MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).


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