• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MAFF Recombinant antibody (85186-3-RR)

    WB analysis using 85186-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAFF Recombinant antibody (85186-3-RR)

    WB analysis using 85186-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAFF Recombinant antibody (85186-3-RR)

    IF Staining of U2OS using 85186-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAFF antibody (85186-3-RR, Clone: 242859F9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MAFF Recombinant antibody

MAFF Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85186-3-RR
Clone No.242859F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, U-Maf, U MAF, 242859F9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, mouse placenta tissue, HuH-7 cells, L02 cells, 4T1 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85186-3-RR targets MAFF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAFF fusion protein Ag3460 相同性解析による交差性が予測される生物種
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
Calculated molecular weight 17 aa, 8 kDa
Observed molecular weight 18-23 kDa
GenBank accession numberBC015037
Gene Symbol MAFF
Gene ID (NCBI) 23764
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9ULX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

Protocols

Product Specific Protocols
WB protocol for MAFF antibody 85186-3-RRDownload protocol
IF protocol for MAFF antibody 85186-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols