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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAFF Recombinant antibody (85186-3-RR)

    WB analysis using 85186-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAFF Recombinant antibody (85186-3-RR)

    WB analysis using 85186-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAFF Recombinant monoclonal antibody (85186-3-RR)

    IHC staining of human lung cancer using 85186-3-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAFF Recombinant monoclonal antibody (85186-3-RR)

    IHC staining of human lung cancer using 85186-3-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MAFF Recombinant monoclonal antibody (85186-3-RR)

    IHC staining of human ovary cancer using 85186-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAFF Recombinant antibody (85186-3-RR)

    IF Staining of U2OS using 85186-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAFF antibody (85186-3-RR, Clone: 242859F9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MAFF Recombinant monoclonal antibody

MAFF Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85186-3-RR
Clone No.242859F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242859F9, U MAF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, mouse placenta tissue, HuH-7 cells, L02 cells, 4T1 cells
Positive IHC detected inhuman lung cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85186-3-RR targets MAFF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3460

Product name: Recombinant human MAFF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-164 aa of BC015037

Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS

相同性解析による交差性が予測される生物種
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
Calculated molecular weight 17 aa, 8 kDa
Observed molecular weight 18-23 kDa
GenBank accession numberBC015037
Gene Symbol MAFF
Gene ID (NCBI) 23764
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

Protocols

Product Specific Protocols
IF protocol for MAFF antibody 85186-3-RRDownload protocol
IHC protocol for MAFF antibody 85186-3-RRDownload protocol
WB protocol for MAFF antibody 85186-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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