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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAFF Recombinant monoclonal antibody (85186-3-RR)

    WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAFF Recombinant monoclonal antibody (85186-3-RR)

    WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAFF Recombinant monoclonal antibody (85186-3-RR)

    IHC staining of human lung cancer using 85186-3-RR (same clone as 85186-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAFF Recombinant monoclonal antibody (85186-3-RR)

    IHC staining of human lung cancer using 85186-3-RR (same clone as 85186-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MAFF Recombinant monoclonal antibody (85186-3-RR)

    IHC staining of human ovary cancer using 85186-3-RR (same clone as 85186-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAFF Recombinant monoclonal antibody (85186-3-RR)

    IF Staining of U2OS using 85186-3-RR (same clone as 85186-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAFF antibody (85186-3-RR, Clone: 242859F9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

MAFF Recombinant monoclonal antibody, PBS Only

MAFF Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85186-3-PBS
Clone No.242859F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242859F9, U MAF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85186-3-PBS targets MAFF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3460

Product name: Recombinant human MAFF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-164 aa of BC015037

Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS

相同性解析による交差性が予測される生物種
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
Calculated molecular weight 17 aa, 8 kDa
Observed molecular weight 18-23 kDa
GenBank accession numberBC015037
Gene Symbol MAFF
Gene ID (NCBI) 23764
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULX9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

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