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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Liprin Alpha 1 Polyclonal antibody

Liprin Alpha 1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14175

Synonyms

FLJ41337, FLJ42630, FLJ43474, LAR interacting protein 1, LIP 1, LIP.1, LIP1, LIPRIN, Liprin alpha 1, PPFIA1

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL647-14175

    FC experiment of HeLa using CL647-14175

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Liprin Alpha 1 (CL647-14175) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPFIA1 Ready-To-Use IHC Kit. PPFIA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14175-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-14175 targets Liprin Alpha 1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Liprin Alpha 1 fusion protein Ag5381 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Calculated molecular weight 1202 aa, 136 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC034046
Gene symbol PPFIA1
Gene ID (NCBI) 8500
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 Liprin Alpha 1 antibody CL647-14175Download protocol
Standard Protocols
Click here to view our Standard Protocols