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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated LZTFL1 Monoclonal antibody

LZTFL1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C1F6

Cat no : CL488-68281

Synonyms

FLJ36386, LZTFL1

Validation Data Gallery

Applications:
  • FC experiment of Jurkat using CL488-68281

    FC experiment of Jurkat using CL488-68281

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LZTFL1 (CL488-68281, Clone:2C1F6) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 68281-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-68281 targets LZTFL1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LZTFL1 fusion protein Ag10733 相同性解析による交差性が予測される生物種
Full Name leucine zipper transcription factor-like 1
Calculated molecular weight 299 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC025988
Gene symbol LZTFL1
Gene ID (NCBI) 54585
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 LZTFL1 antibody CL488-68281Download protocol
Standard Protocols
Click here to view our Standard Protocols