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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LSM3 Polyclonal antibody (15879-1-AP)

    WB analysis using 15879-1-AP (same clone as 15879-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15879-1-AP (LSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of mouse testis using 15879-1-AP (same clone as 15879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of rat testis using 15879-1-AP (same clone as 15879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of mouse testis using 15879-1-AP (same clone as 15879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of rat testis using 15879-1-AP (same clone as 15879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using LSM3 Polyclonal antibody (15879-1-AP)

    IF Staining of NIH/3T3 using 15879-1-AP (same clone as 15879-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using LSM3 antibody (15879-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

LSM3 Polyclonal antibody, PBS Only

LSM3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15879-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

YLR438C, USS2, U6 snRNA-associated Sm-like protein LSm3, SMX4, MDS017

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15879-1-PBS targets LSM3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8641

Product name: Recombinant human LSM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC007055

Sequence: MADDVDQQQTTNTVEEPLDLIRLSLDERIYVKMRNDRELRGRLHAYDQHLNMILGDVEETVTTIEIDEETYEEIYKSTKRNIPMLFVRGDGVVLVAPPLRVG

相同性解析による交差性が予測される生物種
Full Name LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Calculated molecular weight 102 aa, 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC007055
Gene Symbol LSM3
Gene ID (NCBI) 27258
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP62310
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LSM3, as a U6 snRNA-associated Sm-like protein, plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)

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