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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LSM3 Polyclonal antibody (15879-1-AP)

    WB analysis using 15879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15879-1-AP (LSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of mouse testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of rat testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of mouse testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using LSM3 Polyclonal antibody (15879-1-AP)

    IHC staining of rat testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using LSM3 Polyclonal antibody (15879-1-AP)

    IF Staining of NIH/3T3 using 15879-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using LSM3 antibody (15879-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

LSM3 Polyclonal antibody

LSM3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15879-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

YLR438C, USS2, U6 snRNA-associated Sm-like protein LSm3, SMX4, MDS017

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15879-1-AP targets LSM3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM3 fusion protein Ag8641 相同性解析による交差性が予測される生物種
Full Name LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Calculated molecular weight 102 aa, 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC007055
Gene Symbol LSM3
Gene ID (NCBI) 27258
RRIDAB_3669222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62310
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LSM3, as a U6 snRNA-associated Sm-like protein, plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)

Protocols

Product Specific Protocols
WB protocol for LSM3 antibody 15879-1-APDownload protocol
IHC protocol for LSM3 antibody 15879-1-APDownload protocol
IF protocol for LSM3 antibody 15879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols