Validation Data Gallery
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Tested Applications
| Positive WB detected in | Daudi cells, U-87 MG cells, L02 cells |
| Positive IF/ICC detected in | L02 cells |
| Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83693-3-RR targets LRRC47 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | LRRC47 fusion protein Ag19736 相同性解析による交差性が予測される生物種 |
| Full Name | leucine rich repeat containing 47 |
| Calculated molecular weight | 583 aa, 63 kDa |
| Observed molecular weight | 63 kDa |
| GenBank accession number | BC031301 |
| Gene Symbol | LRRC47 |
| Gene ID (NCBI) | 57470 |
| RRID | AB_3671296 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q8N1G4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LRRC47 antibody 83693-3-RR | Download protocol |
| IF protocol for LRRC47 antibody 83693-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |