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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRRC47 Recombinant monoclonal antibody (83693-3-RR)

    WB analysis using 83693-3-RR (same clone as 83693-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using LRRC47 Recombinant monoclonal antibody (83693-3-RR)

    IF Staining of L02 using 83693-3-RR (same clone as 83693-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using LRRC47 Recombinant monoclonal antibody (83693-3-RR)

    FC experiment of U2OS using 83693-3-RR (same clone as 83693-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83693-3-RR

    Affinity and Kinetic Characterization of 83693-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

LRRC47 Recombinant monoclonal antibody, PBS Only

LRRC47 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83693-3-PBS
Clone No.240741A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240741A6, KIAA1185, Leucine-rich repeat-containing protein 47

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83693-3-PBS targets LRRC47 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19736

Product name: Recombinant human LRRC47 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-583 aa of BC031301

Sequence: RDKRLEKMVSGCQTRSILEYLRVGGRGGGKGKGRAEGSEKEESRRKRRERKQRREGGDGEEQDVGDAGRLLLRVLHVSENPVPLTVRVSPEVRDVRPYIVGAVVRGMDLQPGNALKRFLTSQTKLHEDLCEKRTAATLATHELRAVKGPLLYCARPPQDLKIVPLGRKEAKAKELVRQLQLEAEEQRKQKKRQSVSGLHRYLHLLDGNENYPCLVDADGDVISFPPITNSEKTKVKKTTSDLFLEVTSATSLQICKDVMDALILKMAEMKKYTLENKEEGSLSDTEADAVSGQLPDPTTNPSAGKDGPSLLVVEQVRVVDLEGSLKVVYPSKADLATAPPHVTVVR

相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 47
Calculated molecular weight 583 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC031301
Gene Symbol LRRC47
Gene ID (NCBI) 57470
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ8N1G4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

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