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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using LMCD1 Monoclonal antibody (67834-1-Ig)

    WB analysis of U2OS using 67834-1-Ig (same clone as 67834-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    WB analysis of human placenta using 67834-1-Ig (same clone as 67834-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of rat skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of rat skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon cancer using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon cancer using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LMCD1 Monoclonal antibody (67834-1-Ig)

    IF Staining of MCF-7 using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMCD1 antibody (67834-1-Ig, Clone: 2A7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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LMCD1 Monoclonal antibody, PBS Only

LMCD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67834-1-PBS
Clone No.2A7B3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Dyxin, LMCD1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67834-1-PBS targets LMCD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LMCD1 fusion protein Ag28331 相同性解析による交差性が予測される生物種
Full Name LIM and cysteine-rich domains 1
Calculated molecular weight 41 kDa
Observed molecular weight~40 kDa
GenBank accession numberBC000646
Gene Symbol LMCD1
Gene ID (NCBI) 29995
RRIDAB_2918596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZU5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LIM and cysteine-rich domains-1 (LMCD1) is a member of the LIM protein family, which contains an N-terminal cysteine-rich region, two C-terminal LIM domains and a central PET (Prickle, Espinas, and Testin) domain. LMCD1 has been reported in cardiac tissues and lung acting as a transcriptional repressor for GATA6. The mutations of LMCD1 promote cell migration and tumor metastasis in hepatocellular carcinoma. (PMID: 31501411) It has calculated molecular weight around 41kDa.