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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LMCD1 Monoclonal antibody

LMCD1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7B3

Cat no : 67834-1-Ig

Synonyms

Dyxin, LMCD1

Validation Data Gallery

Applications:
  • WB analysis of U2OS using 67834-1-Ig

    WB analysis of U2OS using 67834-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 67834-1-Ig

    WB analysis of human placenta using 67834-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 67834-1-Ig

    IHC staining of human colon using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of mouse skeletal muscle using 67834-1-Ig

    IHC staining of mouse skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of mouse skeletal muscle using 67834-1-Ig

    IHC staining of mouse skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of human colon cancer using 67834-1-Ig

    IHC staining of human colon cancer using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of human colon cancer using 67834-1-Ig

    IHC staining of human colon cancer using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of rat skeletal muscle using 67834-1-Ig

    IHC staining of rat skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of rat skeletal muscle using 67834-1-Ig

    IHC staining of rat skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of human colon using 67834-1-Ig

    IHC staining of human colon using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IF Staining of MCF-7 using 67834-1-Ig

    IF Staining of MCF-7 using 67834-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMCD1 antibody (67834-1-Ig, Clone: 2A7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inU2OS cells, human placenta tissue
Positive IHC detected inhuman colon tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy LMCD1 Ready-To-Use IHC Kit. LMCD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67834-1-Ig targets LMCD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LMCD1 fusion protein Ag28331 相同性解析による交差性が予測される生物種
Full Name LIM and cysteine-rich domains 1
Calculated molecular weight 41 kDa
Observed molecular weight~40 kDa
GenBank accession numberBC000646
Gene symbol LMCD1
Gene ID (NCBI) 29995
RRIDAB_2918596
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIM and cysteine-rich domains-1 (LMCD1) is a member of the LIM protein family, which contains an N-terminal cysteine-rich region, two C-terminal LIM domains and a central PET (Prickle, Espinas, and Testin) domain. LMCD1 has been reported in cardiac tissues and lung acting as a transcriptional repressor for GATA6. The mutations of LMCD1 promote cell migration and tumor metastasis in hepatocellular carcinoma. (PMID: 31501411) It has calculated molecular weight around 41kDa.

Protocols

Product Specific Protocols
WB protocol for LMCD1 antibody 67834-1-IgDownload protocol
IHC protocol for LMCD1 antibody 67834-1-IgDownload protocol
IF protocol for LMCD1 antibody 67834-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols