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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIX1L Polyclonal antibody (25557-1-AP)

    WB analysis using 25557-1-AP (same clone as 25557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25557-1-AP (LIX1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25557-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using LIX1L Polyclonal antibody (25557-1-AP)

    IF Staining of K-562 using 25557-1-AP (same clone as 25557-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using LIX1L antibody (25557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25557-1-PBS in a different storage buffer formulation.

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LIX1L Polyclonal antibody, PBS Only

LIX1L Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25557-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25557-1-PBS targets LIX1L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIX1L fusion protein Ag22209 相同性解析による交差性が予測される生物種
Full Name Lix1 homolog (mouse)-like
Calculated molecular weight 337 aa, 37 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC035727
Gene Symbol LIX1L
Gene ID (NCBI) 128077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVB5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Limb expression 1-like protein (LIX1L) is a putative RNA binding protein that contains a double-stranded RNA-binding motif. LIX1L is highly expressed in various cancer tissues.