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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIX1L Polyclonal antibody (25557-1-AP)

    WB analysis using 25557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25557-1-AP (LIX1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using LIX1L Polyclonal antibody (25557-1-AP)

    IF Staining of K-562 using 25557-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using LIX1L antibody (25557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

LIX1L Polyclonal antibody

LIX1L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25557-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

LIX1-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, SH-SY5Y cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25557-1-AP targets LIX1L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIX1L fusion protein Ag22209 相同性解析による交差性が予測される生物種
Full Name Lix1 homolog (mouse)-like
Calculated molecular weight 337 aa, 37 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC035727
Gene Symbol LIX1L
Gene ID (NCBI) 128077
RRIDAB_3669484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Limb expression 1-like protein (LIX1L) is a putative RNA binding protein that contains a double-stranded RNA-binding motif. LIX1L is highly expressed in various cancer tissues.

Protocols

Product Specific Protocols
WB protocol for LIX1L antibody 25557-1-APDownload protocol
IF protocol for LIX1L antibody 25557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols