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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LIV-1/ZIP6 Polyclonal antibody (14236-1-AP)

    WB analysis of HeLa using 14236-1-AP (same clone as 14236-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LIV-1/ZIP6 Polyclonal antibody (14236-1-AP)

    WB analysis using 14236-1-AP (same clone as 14236-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LIV-1/ZIP6 Polyclonal antibody (14236-1-AP)

    WB analysis of HeLa using 14236-1-AP (same clone as 14236-1-PBS)

    WB result of LIV-1/ZIP6 antibody (14236-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIV-1/ZIP6 transfected HeLa cells. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using LIV-1/ZIP6 Polyclonal antibody (14236-1-AP)

    WB analysis of MCF-7 using 14236-1-AP (same clone as 14236-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using LIV-1/ZIP6 Polyclonal antibody (14236-1-AP)

    IHC staining of human pancreas cancer using 14236-1-AP (same clone as 14236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using LIV-1/ZIP6 Polyclonal antibody (14236-1-AP)

    IHC staining of human pancreas cancer using 14236-1-AP (same clone as 14236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

LIV-1/ZIP6 Polyclonal antibody, PBS Only

LIV-1/ZIP6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14236-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14236-1-PBS targets LIV-1/ZIP6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIV-1/ZIP6 fusion protein Ag5478 相同性解析による交差性が予測される生物種
Full Name solute carrier family 39 (zinc transporter), member 6
Calculated molecular weight 85 kDa
Observed molecular weight 85-100 kDa, 48 and 32 kDa
GenBank accession numberBC039498
Gene Symbol SLC39A6/ZIP6
Gene ID (NCBI) 25800
RRIDAB_10596924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13433
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The zinc transporter LIV-1 (also known as SLC39A6 or ZIP6) was originally identified as an estrogen-induced gene in breast cancer cells. Elevated LIV-1 expression is reportedly related to cancer progression in various types of cancer, including breast, prostate, pancreatic, cervical and liver cancers. While correlation of LIV-1 and prognosis may vary. LIV 1 has 3 isoforms with MW of 85-100 (glycosylation and phosphorylation), 48 and 32 kDa while migrates as different bands in different lysates in SDS-PAGE.