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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis of HUVEC using 17658-1-AP (same clone as 17658-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis of mouse testis using 17658-1-AP (same clone as 17658-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis using 17658-1-AP (same clone as 17658-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis of human brain using 17658-1-AP (same clone as 17658-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human testis using 17658-1-AP (same clone as 17658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human testis using 17658-1-AP (same clone as 17658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human spleen using 17658-1-AP (same clone as 17658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human spleen using 17658-1-AP (same clone as 17658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human ovary using 17658-1-AP (same clone as 17658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human ovary using 17658-1-AP (same clone as 17658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17658-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LGR6 Polyclonal antibody, PBS Only

LGR6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17658-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

VTS20631, UNQ6427/PRO21331, Leucine-rich repeat-containing G-protein coupled receptor 6, GPCR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17658-1-PBS targets LGR6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11909

Product name: Recombinant human LGR6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC047905

Sequence: MGRPRLTLVCQVSIIISARDLSMNNLTELQPGLFHHLRFLEELRLSGNHLSHIPGQAFSGLYSLKILMLQNNQLGGIPAEALWELPSLQSLRLDANLISLVPERSFEGLSSLRHLWLDDNALTEIPVRALNNLPALQAMTLALNRISHIPDYAFQNLTSLVVLHLHNNRIQHLGTHSFEGLHNLETLDLNYNKLQEFPVAIRTLGRLQELGFHNNNIKAIPEKAFMGNPLLQTIHFYDNPIQFVGRSAFQYLPKLHTLSLNGAMDIQEFPDLKGTTSLEILTLTRAGIRLLPSGMCQQLPRLRVLELSHNQIEELPSLHRCQKLEEIGLQHNRIWEIGADTFSQLSSLQAL

相同性解析による交差性が予測される生物種
Full Name leucine-rich repeat-containing G protein-coupled receptor 6
Calculated molecular weight 915 aa, 99 kDa
Observed molecular weight 104 kDa
GenBank accession numberBC047905
Gene Symbol LGR6
Gene ID (NCBI) 59352
RRIDAB_2135163
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBX8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)

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