Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, human brain tissue, mouse testis tissue, HeLa cells, LNCaP cells, PC-3 cells |
| Positive IHC detected in | human testis tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| ELISA | See 1 publications below |
Product Information
17658-1-AP targets LGR6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11909 Product name: Recombinant human LGR6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC047905 Sequence: MGRPRLTLVCQVSIIISARDLSMNNLTELQPGLFHHLRFLEELRLSGNHLSHIPGQAFSGLYSLKILMLQNNQLGGIPAEALWELPSLQSLRLDANLISLVPERSFEGLSSLRHLWLDDNALTEIPVRALNNLPALQAMTLALNRISHIPDYAFQNLTSLVVLHLHNNRIQHLGTHSFEGLHNLETLDLNYNKLQEFPVAIRTLGRLQELGFHNNNIKAIPEKAFMGNPLLQTIHFYDNPIQFVGRSAFQYLPKLHTLSLNGAMDIQEFPDLKGTTSLEILTLTRAGIRLLPSGMCQQLPRLRVLELSHNQIEELPSLHRCQKLEEIGLQHNRIWEIGADTFSQLSSLQAL 相同性解析による交差性が予測される生物種 |
| Full Name | leucine-rich repeat-containing G protein-coupled receptor 6 |
| Calculated molecular weight | 915 aa, 99 kDa |
| Observed molecular weight | 104 kDa |
| GenBank accession number | BC047905 |
| Gene Symbol | LGR6 |
| Gene ID (NCBI) | 59352 |
| RRID | AB_2135163 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBX8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LGR6 antibody 17658-1-AP | Download protocol |
| WB protocol for LGR6 antibody 17658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.
| ||
Int J Biol Macromol Study on the mechanism of macrophages activated by phosphoesterified rehmanniae polysaccharide on human gastric cancer cells | ||
Mol Ther Oncolytics Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer. | ||
Proc Natl Acad Sci U S A The Maresin 1-LGR6 axis decreases respiratory syncytial virus-induced lung inflammation |
