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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Galectin-4 Monoclonal antibody

Galectin-4 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat no : 66686-1-Ig

Synonyms

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

Validation Data Gallery

Applications:
  • WB analysis of HT-29 using 66686-1-Ig

    WB analysis of HT-29 using 66686-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human ileum using 66686-1-Ig

    WB analysis of human ileum using 66686-1-Ig

    human ileum tissue were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon cancer using 66686-1-Ig

    IHC staining of human colon cancer using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon cancer using 66686-1-Ig

    IHC staining of human colon cancer using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IF Staining of human colon cancer using 66686-1-Ig

    IF Staining of human colon cancer using 66686-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human colon cancer using 66686-1-Ig

    IF Staining of human colon cancer using 66686-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HT-29 using 66686-1-Ig

    FC experiment of HT-29 using 66686-1-Ig

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Galectin-4 (66686-1-Ig, Clone:3C2G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHT-29 cells, human ileum tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue
Positive FC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy LGALS4 Ready-To-Use IHC Kit. LGALS4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:5000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66686-1-Ig targets Galectin-4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Galectin-4 fusion protein Ag4049 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 4
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC034750
Gene symbol LGALS4
Gene ID (NCBI) 3960
RRIDAB_2882040
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

Protocols

Product Specific Protocols
WB protocol for Galectin-4 antibody 66686-1-IgDownload protocol
IHC protocol for Galectin-4 antibody 66686-1-IgDownload protocol
IF protocol for Galectin-4 antibody 66686-1-IgDownload protocol
FC protocol for Galectin-4 antibody 66686-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols