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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using Galectin-4 Monoclonal antibody (66686-1-Ig)

    WB analysis of HT-29 using 66686-1-Ig (same clone as 66686-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ileum tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    WB analysis of human ileum using 66686-1-Ig (same clone as 66686-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Galectin-4 Monoclonal antibody (66686-1-Ig)

    IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

Galectin-4 Monoclonal antibody, PBS Only

Galectin-4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66686-1-PBS
Clone No.3C2G3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

3C2G3, Antigen NY CO 27, Antigen NY-CO-27, Gal 4, GAL4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66686-1-PBS targets Galectin-4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4049

Product name: Recombinant human LGALS4,GAL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC034750

Sequence: MAYVPAPGYQPTYNPTLPYYQPIPGGLNVGMSVYIQGVASEHMKRFFVNFVVGQDPGSDVAFHFNPRFDGWDKVVFNTLQGGKWGSEERKRSMPFKKGAAFELVFIVLAEHYKVVVNGNPFYEYGHRLPLQMVTHLQVDGDLQLQSINFIGGQPLRPQGPPMMPPYPGPGHCHQQLNSLPTMEGPPTFNPPVPYFGRLQGGLTARRTIIIKGYVPPTGKSFAINFKVGSSGDIALHINPRMGNGTVVRNSLLNGSWGSEEKKITHNPFGPGQFFDLSIRCGLDRFKVYANGQHLFDFAHRLSAFQRVDTLEIQGDVTLSYVQI

相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 4
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC034750
Gene Symbol Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2882040
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP56470
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

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