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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of 3T3-L1 cells using LEP Recombinant antibody (84072-5-RR)

    IF Staining of 3T3-L1 using 84072-5-RR

    Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using LEP antibody (84072-5-RR, Clone: 240720A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using LEP Recombinant antibody (84072-5-RR)

    FC experiment of HeLa using 84072-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HepG2 cells using LEP Recombinant antibody (84072-5-RR)

    FC experiment of HepG2 using 84072-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84072-5-RR

    Affinity and Kinetic Characterization of 84072-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84072-5-RR against Human LEP were performed. The affinity constant is 35.3 nM.

Leptin Recombinant monoclonal antibody

Leptin Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84072-5-RR
Clone No.240720A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

LEP, 240720A6, OB, Obese protein, Obesity factor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected in3T3-L1 cells
Positive FC (Intra) detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84072-5-RR targets Leptin in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0834

Product name: Recombinant Human Leptin protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 22-167 aa of BC060830

Sequence: VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

相同性解析による交差性が予測される生物種
Full Name leptin
Calculated molecular weight 167 aa, 19 kDa
GenBank accession numberBC060830
Gene Symbol Leptin
Gene ID (NCBI) 3952
RRIDAB_3671642
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP41159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leptin is a product of the obese (ob) gene and, following synthesis and secretion from fat cells in white adipose tissue, binds to and activates its cognate receptor, the leptin receptor (LEP-R) (PMID: 34084149). Still, smaller quantities have been detected in other body tissues, including the brown adipose tissue (BAT), placenta, fetal tissue, stomach, muscles, bone marrow, teeth, and brain (PMID:7568067, PMID: 12086939). Leptin circulates in the blood in both free and protein-bound forms, where the free form of leptin is the biologically active form (PMID: 12086939). Leptin can enter the central nervous system (CNS) (in the area of the choroid plexus) by receptor-mediated transport (PMID: 29254602). Leptin secretion is higher in subcutaneous than in visceral adipose tissue (PMID: 11133069, PMID: 14726444).

Protocols

Product Specific Protocols
IF protocol for Leptin antibody 84072-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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