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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of 3T3-L1 cells using Leptin Recombinant antibody (84072-5-RR)

    IF Staining of 3T3-L1 using 84072-5-RR (same clone as 84072-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using LEP antibody (84072-5-RR, Clone: 240720A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84072-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Leptin Recombinant antibody (84072-5-RR)

    FC experiment of HeLa using 84072-5-RR (same clone as 84072-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84072-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Leptin Recombinant antibody (84072-5-RR)

    FC experiment of HepG2 using 84072-5-RR (same clone as 84072-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84072-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84072-5-RR

    Affinity and Kinetic Characterization of 84072-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84072-5-RR against Human LEP were performed. The affinity constant is 35.3 nM.

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Leptin Recombinant antibody, PBS Only

Leptin Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84072-5-PBS
Clone No.240720A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

LEP, 240720A6, OB, Obese protein, Obesity factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84072-5-PBS targets Leptin in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Leptin fusion protein Eg0834 相同性解析による交差性が予測される生物種
Full Name leptin
Calculated molecular weight 167 aa, 19 kDa
GenBank accession numberBC060830
Gene Symbol Leptin
Gene ID (NCBI) 3952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP41159
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Leptin is a product of the obese (ob) gene and, following synthesis and secretion from fat cells in white adipose tissue, binds to and activates its cognate receptor, the leptin receptor (LEP-R) (PMID: 34084149). Still, smaller quantities have been detected in other body tissues, including the brown adipose tissue (BAT), placenta, fetal tissue, stomach, muscles, bone marrow, teeth, and brain (PMID:7568067, PMID: 12086939). Leptin circulates in the blood in both free and protein-bound forms, where the free form of leptin is the biologically active form (PMID: 12086939). Leptin can enter the central nervous system (CNS) (in the area of the choroid plexus) by receptor-mediated transport (PMID: 29254602). Leptin secretion is higher in subcutaneous than in visceral adipose tissue (PMID: 11133069, PMID: 14726444).