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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LCMT2 Recombinant antibody (83073-2-RR)

    IF Staining of HepG2 using 83073-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCMT2 antibody (83073-2-RR, Clone: 230405H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using LCMT2 Recombinant antibody (83073-2-RR)

    FC experiment of HeLa using 83073-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LCMT2 Recombinant antibody (83073-2-RR, Clone:230405H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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LCMT2 Recombinant antibody

LCMT2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83073-2-RR
Clone No.230405H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

tRNA yW-synthesizing protein 4, tRNA wybutosine-synthesizing protein 4, PPM2, KIAA0547, EC:2.1.1.290

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83073-2-RR targets LCMT2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LCMT2 fusion protein Ag11145 相同性解析による交差性が予測される生物種
Full Name leucine carboxyl methyltransferase 2
Calculated molecular weight aa, 76 kDa
GenBank accession numberBC015949
Gene Symbol LCMT2
Gene ID (NCBI) 9836
RRIDAB_3670792
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LCMT2, also known as tRNA wybutosine-synthesizing protein 4 (TYW4), is an S-adenosylmethionine (SAM)-dependent enzyme that catalyzes the final step of yW biosynthesis, methylation and methoxycarbonylation.LCMT2 has a conserved leucine carboxyl methyltransferase (LCM) domain in its N-terminal region (PMID: 16642040).

Protocols

Product Specific Protocols
IF protocol for LCMT2 antibody 83073-2-RRDownload protocol
FC protocol for LCMT2 antibody 83073-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols