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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LCMT2 Recombinant monoclonal antibody (83073-2-RR)

    IF Staining of HepG2 using 83073-2-RR (same clone as 83073-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCMT2 antibody (83073-2-RR, Clone: 230405H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using LCMT2 Recombinant monoclonal antibody (83073-2-RR)

    FC experiment of HeLa using 83073-2-RR (same clone as 83073-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LCMT2 Recombinant antibody (83073-2-RR, Clone:230405H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83073-2-PBS

    Affinity and Kinetic Characterization of 83073-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83073-2-PBS against Human LCMT2 was performed. The affinity constant is 68.8 nM.

LCMT2 Recombinant monoclonal antibody, PBS Only

LCMT2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83073-2-PBS
Clone No.230405H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230405H8, EC:2.1.1.290, KIAA0547, PPM2, tRNA wybutosine-synthesizing protein 4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83073-2-PBS targets LCMT2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11145

Product name: Recombinant human LCMT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 340-686 aa of BC015949

Sequence: VFPASVVSSEGQVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLLSRDCDSEWKGSQIGSCGTGVQWDGRLYHTMTRLSESRVLVLGGRLSPVSPALGVLQLHFFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQNQEYLFVYGGRSVVEPVLSDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLNSVLFLRPISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLSSEYQIDTTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSLSAGQ

相同性解析による交差性が予測される生物種
Full Name leucine carboxyl methyltransferase 2
Calculated molecular weight aa, 76 kDa
GenBank accession numberBC015949
Gene Symbol LCMT2
Gene ID (NCBI) 9836
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO60294
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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