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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated LARP1 Monoclonal antibody

LARP1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E9F2

Cat no : CL555-67810

Synonyms

KIAA0731, La related protein 1, LARP, LARP1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL555-67810

    IF Staining of HepG2 using CL555-67810

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 LARP1 antibody (CL555-67810, Clone: 2E9F2 ) at dilution of 1:400.

  • FC experiment of HepG2 using CL555-67810

    FC experiment of HepG2 using CL555-67810

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human LARP1 (CL555-67810, Clone:2E9F2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67810-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL555-67810 targets LARP1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LARP1 fusion protein Ag29993 相同性解析による交差性が予測される生物種
Full Name La ribonucleoprotein domain family, member 1
Calculated molecular weight 1096 aa, 124 kDa
Observed molecular weight140-150 kDa
GenBank accession numberBC033856
Gene symbol LARP1
Gene ID (NCBI) 23367
RRIDAB_2919705
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LARP1, full named as La-related Protein 1, is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. (PMID: 32286153)It's has been proposed to function downstream of mTORC1 to regulate the translation of 5'TOP mRNAs such as those encoding ribosome proteins (RP). (PMID: 28650797) LARP1 has 2 isoforms with MW of 123-150KDa. With CL647, immunoflorecence detected location in cytoplasm.

Protocols

Product Specific Protocols
IF protocol for CL555 LARP1 antibody CL555-67810Download protocol
FC protocol for CL555 LARP1 antibody CL555-67810Download protocol
Standard Protocols
Click here to view our Standard Protocols