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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using LANCL1 Polyclonal antibody (12647-1-AP)

    WB analysis of mouse testis using 12647-1-AP (same clone as 12647-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12647-1-AP (LANCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LANCL1 Polyclonal antibody (12647-1-AP)

    IHC staining of mouse brain using 12647-1-AP (same clone as 12647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12647-1-AP (LANCL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using LANCL1 Polyclonal antibody (12647-1-AP)

    IF Staining of U-251 using 12647-1-AP (same clone as 12647-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LANCL1 antibody (12647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12647-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12647-1-PBS targets LANCL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LANCL1 fusion protein Ag3361 相同性解析による交差性が予測される生物種
Full Name LanC lantibiotic synthetase component C-like 1 (bacterial)
Calculated molecular weight 399 aa, 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC028685
Gene Symbol LANCL1
Gene ID (NCBI) 10314
RRIDAB_10638314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43813
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.