Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
12647-1-AP targets LANCL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3361 Product name: Recombinant human LANCL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-399 aa of BC028685 Sequence: TKRSITFLCGDAGPLAVAAVLYHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQQICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKLHSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLCDAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGEHGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL 相同性解析による交差性が予測される生物種 |
| Full Name | LanC lantibiotic synthetase component C-like 1 (bacterial) |
| Calculated molecular weight | 399 aa, 45 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC028685 |
| Gene Symbol | LANCL1 |
| Gene ID (NCBI) | 10314 |
| RRID | AB_10638314 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LANCL1 antibody 12647-1-AP | Download protocol |
| IHC protocol for LANCL1 antibody 12647-1-AP | Download protocol |
| WB protocol for LANCL1 antibody 12647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway.
| ||
J Lipid Res Lanthionine synthetase C-like protein 2 (LanCL2) is Important for Adipogenic Differentiation.
| ||
Front Microbiol The Endogenous Alterations of the Gut Microbiota and Feces Metabolites Alleviate Oxidative Damage in the Brain of LanCL1 Knockout Mice.
| ||
Exp Neurol LanCL1 protects developing neurons from long-term isoflurane anesthesia-induced neurotoxicity |