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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMTOR4 Polyclonal antibody (32958-1-AP)

    WB analysis using 32958-1-AP (same clone as 32958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32958-1-AP (LAMTOR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32958-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using LAMTOR4 Polyclonal antibody (32958-1-AP)

    IP experiment of HeLa using 32958-1-AP (same clone as 32958-1-PBS)

    IP result of anti-LAMTOR4 (IP:32958-1-AP, 4ug; Detection:32958-1-AP 1:1000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 32958-1-PBS in a different storage buffer formulation.

LAMTOR4 Polyclonal antibody, PBS Only

LAMTOR4 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 32958-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

C7orf59, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4, Ragulator complex protein LAMTOR4, Ragulator complex protein LAMTOR4, N-terminally processed

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32958-1-PBS targets LAMTOR4 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag39105

Product name: Recombinant human C7orf59 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC130553

Sequence: MTSALTQGLERIPDQLGYLVLSEGAVLASSGDLENDEQAASAISELVSTACGFRLHRGMNVPFKRLSVVFGEHTLLVTVSGQRVFVVKRQNRGREPIDV

相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 59
Calculated molecular weight11 kDa
Observed molecular weight11 kDa
GenBank accession numberBC130553
Gene Symbol C7orf59
Gene ID (NCBI) 389541
RRIDAB_3742805
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ0VGL1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 (LAMTOR4) is a protein belonging to the LAMTOR family and believed to be involved in cell cycle, proliferation, and growth (PMID: 29138253). LAMTOR4 has been reported as a regulator complex that acts as an activator of rapamycin complex 1 (mTORC1) by sensing changes in the levels of amino acids (PMID: 33429026). It might offer a strategy to inhibit tumor progression and metastasis in prostate cancer (PMID: 39125671).

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