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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMTOR4 Polyclonal antibody (32958-1-AP)

    WB analysis using 32958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32958-1-AP (LAMTOR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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LAMTOR4 Polyclonal antibody

LAMTOR4 Polyclonal Antibody for WB, ELISA
Cat No. 32958-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C7orf59, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4, Ragulator complex protein LAMTOR4, Ragulator complex protein LAMTOR4, N-terminally processed

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, PANC-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32958-1-AP targets LAMTOR4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMTOR4 fusion protein Ag39105 相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 59
Calculated molecular weight11 kDa
Observed molecular weight11 kDa
GenBank accession numberBC130553
Gene Symbol C7orf59
Gene ID (NCBI) 389541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ0VGL1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 (LAMTOR4) is a protein belonging to the LAMTOR family and believed to be involved in cell cycle, proliferation, and growth (PMID: 29138253). LAMTOR4 has been reported as a regulator complex that acts as an activator of rapamycin complex 1 (mTORC1) by sensing changes in the levels of amino acids (PMID: 33429026). It might offer a strategy to inhibit tumor progression and metastasis in prostate cancer (PMID: 39125671).

Protocols

Product Specific Protocols
WB protocol for LAMTOR4 antibody 32958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols