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Host

Species Reactivity

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ChromoTek LAG3 VHH, recombinant binding protein for 2x Cys conjugation

Alpaca anti-LAG3 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Cat no : ltCys2

Synonyms

LAG3-Nanobody, LAG3-single domain antibody (sdAb), CD223, FDC, LAG 3, LAG3, LAG-3, lymphocyte activation gene 3, Protein FDC

Validation Data Gallery

  • The affinity of anti-human LAG3 recombinant VHH towards human LAG3 was determined using biolayer interferometry (BLI). Biotinylated, recombinant human LAG3 was immobilized on Streptavidin biosensors and assayed with 6.3 to 50 nM of CoraLite® Plus 647-conjugated LAG3 VHH (CL647-lt). Fit indicates a 1:1 binding model fitted to the data.

    The affinity of anti-human LAG3 recombinant VHH towards human LAG3 was determined using biolayer interferometry (BLI). Biotinylated, recombinant human LAG3 was immobilized on Streptavidin biosensors and assayed with 6.3 to 50 nM of CoraLite® Plus 647-conjugated LAG3 VHH (CL647-lt). Fit indicates a 1:1 binding model fitted to the data.

Product Information

Alpaca anti-LAG3 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

ApplicationsConjugation
ReactivityHuman
ConjugateUnconjugated
Type Nanobody
ClassRecombinant
HostAlpaca
Affinityin the picomolar range, below the assay limit (biolayer interferometry)
Molecular Weight15.141 kDa
FormLiquid
Storage Buffer10 mM HEPES, 500 mM NaCl, pH 7.0, 1 mM TCEP, 0.09% sodium azide
Storage ConditionStore at -20°C; Avoid exposure to light. Shipped at dry ice.
BackgroundLAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)