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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LACE1 Polyclonal antibody (30157-1-AP)

    WB analysis using 30157-1-AP (same clone as 30157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30157-1-AP (LACE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LACE1 Polyclonal antibody (30157-1-AP)

    IF Staining of HepG2 using 30157-1-AP (same clone as 30157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LACE1 antibody (30157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using LACE1 Polyclonal antibody (30157-1-AP)

    IF Staining of U2OS using 30157-1-AP (same clone as 30157-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using LACE1 antibody (30157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30157-1-PBS in a different storage buffer formulation.

LACE1 Polyclonal antibody, PBS Only

LACE1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AFG1, AFG1L, AFG1-like ATPase, EC:3.6.-.-, Lactation elevated protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30157-1-PBS targets LACE1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32827

Product name: Recombinant human LACE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-313 aa of BC018445

Sequence: LAQSPLRGRCVGCGAWAAALAPLATAPGKPFWKAYTVQTSESMTPTATSETYLKALAVCHGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSGIDYRKRELPAAGKLYYLTSEADVEAVMDKLFDE

相同性解析による交差性が予測される生物種
Full Name lactation elevated 1
Calculated molecular weight54 kDa
Observed molecular weight54-58 kda
GenBank accession numberBC018445
Gene Symbol LACE1
Gene ID (NCBI) 246269
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WV93
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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