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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LACE1 Polyclonal antibody

LACE1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30157-1-AP

Synonyms

AFG1, LACE1, lactation elevated 1, Lactation elevated protein 1

Validation Data Gallery

Applications:
  • WB analysis using 30157-1-AP

    WB analysis using 30157-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30157-1-AP (LACE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 30157-1-AP

    IF Staining of HepG2 using 30157-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LACE1 antibody (30157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, HuH-7 cells, mouse liver tissue, rat liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30157-1-AP targets LACE1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LACE1 fusion protein Ag32827 相同性解析による交差性が予測される生物種
Full Name lactation elevated 1
Calculated molecular weight54 kDa
Observed molecular weight54-58 kda
GenBank accession numberBC018445
Gene symbol LACE1
Gene ID (NCBI) 246269
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LACE1 antibody 30157-1-APDownload protocol
IF protocol for LACE1 antibody 30157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols