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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated L-VOCC Polyclonal antibody

L-VOCC Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21774

Synonyms

CACH2, CACN2, CACNA1C, CACNL1A1, CaV1.2, CCHL1A1, L VOCC, TS

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-21774

    FC experiment of HeLa using CL488-21774

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human L-VOCC (CL488-21774) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using CL488-21774

    FC experiment of HeLa using CL488-21774

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human L-VOCC (CL488-21774) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 21774-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-21774 targets L-VOCC in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L-VOCC fusion protein Ag16455 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, L type, alpha 1C subunit
Calculated molecular weight 2135 aa, 239 kDa
GenBank accession numberBC146846
Gene symbol CACNA1C
Gene ID (NCBI) 775
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 L-VOCC antibody CL488-21774Download protocol
Standard Protocols
Click here to view our Standard Protocols