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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Kiz Polyclonal antibody (21177-1-AP)

    WB analysis of HeLa using 21177-1-AP (same clone as 21177-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21177-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Kiz Polyclonal antibody (21177-1-AP)

    WB analysis of human brain using 21177-1-AP (same clone as 21177-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21177-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Kiz Polyclonal antibody (21177-1-AP)

    IP experiment of HeLa using 21177-1-AP (same clone as 21177-1-PBS)

    IP result of anti-Kiz (IP:21177-1-AP, 3ug; Detection:21177-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 21177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Kiz Polyclonal antibody (21177-1-AP)

    IHC staining of human testis using 21177-1-AP (same clone as 21177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Kiz Polyclonal antibody (21177-1-AP)

    IHC staining of human testis using 21177-1-AP (same clone as 21177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Kiz Polyclonal antibody (21177-1-AP)

    IF Staining of HeLa using 21177-1-AP (same clone as 21177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red). This data was developed using the same antibody clone with 21177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Kiz Polyclonal antibody (21177-1-AP)

    IF Staining of hTERT-RPE1 using 21177-1-AP (same clone as 21177-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21177-1-PBS in a different storage buffer formulation.

Kiz Polyclonal antibody, PBS Only

Kiz Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21177-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21177-1-PBS targets Kiz in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15454

Product name: Recombinant human NCRNA00153 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 221-570 aa of BC045826

Sequence: PVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD

相同性解析による交差性が予測される生物種
Full Name non-protein coding RNA 153
Calculated molecular weight 673 aa, 75 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC045826
Gene Symbol Kiz
Gene ID (NCBI) 55857
RRIDAB_10755292
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2M2Z5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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