Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 21177-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 21177-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21177-1-AP
IP result of anti-Kiz (IP:21177-1-AP, 3ug; Detection:21177-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human testis using 21177-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 21177-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 21177-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).
IF Staining of hTERT-RPE1 using 21177-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Kiz Polyclonal antibody

Kiz Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21177-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C20orf19, Centrosomal protein kizuna, HT013, KIZ, NCRNA00153, non protein coding RNA 153, PLK1S1, Polo like kinase 1 substrate 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 5 publications below

Product Information

21177-1-AP targets Kiz in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15454 Product name: Recombinant human NCRNA00153 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-570 aa of BC045826 Sequence: PVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD 相同性解析による交差性が予測される生物種
Full Name	non-protein coding RNA 153
Calculated molecular weight	673 aa, 75 kDa
Observed molecular weight	90-100 kDa
GenBank accession number	BC045826
Gene Symbol	Kiz
Gene ID (NCBI)	55857
RRID	AB_10755292
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q2M2Z5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for Kiz antibody 21177-1-AP	Download protocol
IHC protocol for Kiz antibody 21177-1-AP	Download protocol
IP protocol for Kiz antibody 21177-1-AP	Download protocol
WB protocol for Kiz antibody 21177-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Authors - Chun So View Article
human	IF	Science The mechanism of acentrosomal spindle assembly in human oocytes Authors - Tianyu Wu View Article
human	WB,IF	Elife A hierarchical pathway for assembly of the distal appendages that organize primary cilia Authors - Tomoharu Kanie View Article
	IF	Elife Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages Authors - Tomoharu Kanie View Article
human	WB,IF	PLoS One Two cancer cell lines utilize Myosin 10 and the kinesin HSET differentially to maintain mitotic spindle bipolarity Authors - Yang-In Yim View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 21177-1-AP

WB analysis of human brain using 21177-1-AP

IP experiment of HeLa using 21177-1-AP

IHC staining of human testis using 21177-1-AP

IHC staining of human testis using 21177-1-AP

IF Staining of HeLa using 21177-1-AP

IF Staining of hTERT-RPE1 using 21177-1-AP

Kiz Polyclonal antibody

Kiz Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

The mechanism of acentrosomal spindle assembly in human oocytes

A hierarchical pathway for assembly of the distal appendages that organize primary cilia

Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages

Two cancer cell lines utilize Myosin 10 and the kinesin HSET differentially to maintain mitotic spindle bipolarity