Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12503-1-AP (same clone as 12503-1-PBS)
mouse brain tissues (heated at 100°C, unboided, heated at 37°C) were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12503-1-AP (same clone as 12503-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
IP experiment of rat brain using 12503-1-AP (same clone as 12503-1-PBS)
IP result of anti-Kir4.1 (IP:12503-1-AP, 4ug; Detection:12503-1-AP 1:500) with rat brain tissue lysate 4800ug. This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 12503-1-AP (same clone as 12503-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 12503-1-AP (same clone as 12503-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12503-1-AP (same clone as 12503-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12503-1-AP (same clone as 12503-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12503-1-AP (same clone as 12503-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12503-1-AP (same clone as 12503-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.

Kir4.1 Polyclonal antibody, PBS Only

Kir4.1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12503-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12503-1-PBS targets Kir4.1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3182 Product name: Recombinant human KCNJ10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 224-426 aa of BC034036 Sequence: ETIRFSQHAVVASHNGKPCLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTFQVDTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVRISNV 相同性解析による交差性が予測される生物種
Full Name	potassium inwardly-rectifying channel, subfamily J, member 10
Calculated molecular weight	379 aa, 41 kDa
Observed molecular weight	41 kDa
GenBank accession number	BC034036
Gene Symbol	Kir4.1
Gene ID (NCBI)	3766
RRID	AB_2234144
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P78508
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

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サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12503-1-AP (same clone as 12503-1-PBS)

WB analysis of human brain using 12503-1-AP (same clone as 12503-1-PBS)

IP experiment of rat brain using 12503-1-AP (same clone as 12503-1-PBS)

IHC staining of human gliomas using 12503-1-AP (same clone as 12503-1-PBS)